The complete arguments for BLASTALL 2.2.4
- -p
- Program Name [String]
- -d
- Database [String] default = nr
- -i
- Query File [File In] default = stdin
- -e
- Expectation value (E) [Real] default = 10.0
- -m
- alignment view options: 0 = pairwise, 1 = query-anchored showing identities, 2 = query-anchored no identities, 3 = flat query-anchored, show identities, 4 = flat query-anchored, no identities, 5 = query-anchored no identities and blunt ends, 6 = flat query-anchored, no identities and blunt ends, 7 = XML Blast output, 8 = tabular, 9 tabular with comment lines [Integer] default = 0
- -o
- BLAST report Output File [File Out] Optional default = stdout
- -F
- Filter query sequence (DUST with blastn, SEG with others) [String] default = T
- -G
- Cost to open a gap (zero invokes default behavior) [Integer] default = 0
- -E
- Cost to extend a gap (zero invokes default behavior) [Integer] default = 0
- -X
- X dropoff value for gapped alignment (in bits) (zero invokes default behavior) blastn 30, megablast 20, tblastx 0, all others 15 [Integer] default = 0
- -I
- Show GI's in deflines [T/F] default = F
- -q
- Penalty for a nucleotide mismatch (blastn only) [Integer] default = -3
- -r
- Reward for a nucleotide match (blastn only) [Integer] default = 1
- -v
- Number of database sequences to show one-line descriptions for (V) [Integer] default = 500
- -b
- Number of database sequence to show alignments for (B) [Integer] default = 250
- -f
- Threshold for extending hits, default if zero blastp 11, blastn 0, blastx 12, tblastn 13 tblastx 13, megablast 0 [Integer] default = 0
- -g
- Perfom gapped alignment (not available with tblastx) [T/F] default = T
- -Q
- Query Genetic code to use [Integer] default = 1
- -D
- DB Genetic code (for tblast[nx] only) [Integer] default = 1
- -a
- Number of processors to use [Integer] default = 1
- -O
- SeqAlign file [File Out] Optional
- -J
- Believe the query defline [T/F] default = F
- -M
- Matrix [String] default = BLOSUM62
- -W
- Word size, default if zero (blastn 11, megablast 28, all others 3) [Integer] default = 0
- -z
- Effective length of the database (use zero for the real size) [Real] default = 0
- -K
- Number of best hits from a region to keep (off by default, if used a value of 100 is recommended) [Integer] default = 0
- -Y
- Effective length of the search space (use zero for the real size) [Real] default = 0
- -S
- Query strands to search against database (for blast[nx], and tblastx) 3 is both, 1 is top, 2 is bottom [Integer] default = 3
- -T
- Produce HTML output [T/F] default = F
- -l
- Restrict search of database to list of GI's [String] Optional
- -U
- Use lower case filtering of FASTA sequence [T/F] Optional default = F
- -y
- X dropoff value for ungapped extensions in bits (0.0 invokes default behavior) blastn 20, megablast 10, all others 7 [Real] default = 0.0
- -Z
- X dropoff value for final gapped alignment in bits (0.0 invokes default behavior) blastn/megablast 50, tblastx 0, all others 25 [Integer] default = 0
- -R
- PSI-TBLASTN checkpoint file [File In] Optional
- -n
- MegaBlast search [T/F] default = F
- -L
- Location on query sequence [String] Optional
- -A
- Multiple Hits window size, default if zero (blastn/megablast 0, all others 40 [Integer] default = 0
- -w
- Frame shift penalty (OOF algorithm for blastx) [Integer] default = 0
- -t
- Length of the largest intron allowed in tblastn for linking HSPs (0 disables linking) [Integer] default = 0